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  1. Disentangling the diversity patterns of the cave microbiome on different substrates and in different microclimatic conditions (e.g. different temperatures), and identifying the possible commonalities with surface microbiomes (e.g. soil, water).

  2. Decoding gut microbiome diversity in oligotrophic and chemoautotrophic cave representatives at different food levels.

  3. Parallelisms and convergences between cave invertebrates and their gut symbionts as an adaptation to low food.

  4. The resistome analysis by identifying new cave bacteria with natural antibiotic resistance and menaquinones (MK, vitamin K) synthesis, and experiments on their possible use for human health.

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